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Do We Need to Bring Anti-malaria Medication and/or Get Any Vaccinations? PDF Print E-mail
Thailand is on the upper end of the "developing country" spectrum and while health, sanitation and medical facilities can vary from locale to locale, most places we will be travelling through will have good to excellent facilites. 

As for vaccinations and malaria prophylactics:

According to the US Center for Disease Control's (CDC) Traveler's Health Advisories:
Most travelers to Thailand are not at risk for malaria and do not need to take an antimalarial drug. Travelers to the border areas of Cambodia, Laos, and Burma should take an antimalarial drug.
The Tour de Thailand does not ride through the risky border areas, so malaria medications should not be necessary.

The US CDC does post recommended vaccinations for Southeast Asia--but most are only valid under special circumstances that you should not be subject to during the Tour de Thailand.  Outside of the standard vaccinations for adults (polio, measels, tetanus) you likely do not need any special vaccinations.  Check out the CDC's Southeast Asia Travel Health page for more information.
 
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Tour de Thailand: A charity bicycle ride by the Tour de Asia Bicycle Touring Co., Ltd.